Gen-Z Scientist Level Up: Workshop Pipetting Untuk Penguatan Deteksi Halal Makanan Viral Dengan Teknik PCR
DOI:
https://doi.org/10.59585/sosisabdimas.v4i3.1430Keywords:
Gen-Z Scientist, Mikropipet, Pipetting, PCR, HalalAbstract
Latar Belakang: Tren makanan viral di media sosial telah meningkatkan perhatian publik terhadap keamanan, mutu, dan kepatuhan halal produk pangan olahan, di mana bahan-bahan tersembunyi sulit diidentifikasi secara visual. Polymerase Chain Reaction (PCR) menjadi metode molekuler yang andal untuk mengautentikasi produk-produk tersebut melalui target DNA spesifik. Tujuan: Untuk meningkatkan keterampilan laboratorium mahasiswa Generasi Z dalam penggunaan mikropipet serta memperdalam pemahaman mereka tentang penerapan PCR dalam autentikasi pangan halal. Metode: Kegiatan pengabdian masyarakat ini melibatkan 30 mahasiswa dalam workshop laboratorium interaktif. Kurikulum mencakup pre-test dan post-test, kuliah interaktif, demonstrasi pipetting, praktik langsung, pelatihan dasar PCR, serta evaluasi keterampilan. Topik utama meliputi teknik pipetting, pemilihan tip, pencegahan kontaminasi, keselamatan kerja laboratorium, ekstraksi DNA, dan autentikasi pangan berbasis PCR. Hasil: Pengetahuan peserta meningkat secara signifikan, dengan rerata skor tes naik dari 61,8 menjadi 88,6. Kemahiran praktis juga meningkat pesat—terutama dalam pengaturan volume, pemasangan tip, mekanika pipetting, dan konsistensi prosedur standar—sehingga 86,7% peserta mencapai peringkat keterampilan "baik". Meskipun demikian, hasil analisis laboratorium harus ditempatkan dalam kerangka pengujian dan sertifikasi halal yang resmi. Kesimpulan: Mengintegrasikan praktik pipetting langsung dengan prinsip dasar PCR secara efektif meningkatkan literasi laboratorium dasar di kalangan ilmuwan muda. Inisiatif ini memperkuat kesiapan mahasiswa untuk menguasai teknologi analisis pangan modern dan mendukung literasi halal berbasis sains.
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